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BACKGROUND@#Skeletal muscle injuries are frequent clinical challenges due to associated fibrosis and disability.Regenerative medicine is an emerging promising strategy for such cases. The aim of this study was to compare between the effects of bone marrow-mesenchymal stem cells (BM-MSCs) versus adipose tissue stromal cells (ADSCs) on regeneration and re-innervation of skeletal muscle laceration injury in Wistar rats at different time intervals. @*METHODS@#Six young male rats were used as a source of allogenic MSCs. Eighty-four adult female rats were divided into: Group I (control), Group II (Untreated Laceration): right gluteal muscle was lacerated and left for spontaneous healing, Group III (BM-MSCs): right gluteal muscle was lacerated with concomitant local intramuscular injection of 1 x 106 BM-MSCs in the lacerated muscle, Group IV (ADSCs): right gluteal muscle was lacerated with concomitant local intramuscular injection of 1 x 106 ADSCs in lacerated muscle. Rats were sacrificed after one, two and eight weeks.Muscles were processed to prepare sections stained with H&E, Mallory’s trichrome and immune-histochemical staining (neurofilament light chain). @*RESULTS@#A significant increase in collagen fibers and failure of re-innervation were noticed in untreated laceration group. BM-MSCs-treated groups showed regeneration of muscle fibers but with increased collagen fibers. Meanwhile, ADSCs showed better regenerative effects evidenced by significant increase in the number of myotubes and significant decrease in collagen deposition. Re-innervation was noticed in MSCs-injected muscles after 8 weeks of laceration. @*CONCLUSION@#Both BM-MSCs and ADSCs improved regeneration of skeletal muscle laceration injury at short- and long-term durations. However, fibrosis was less in ADSCs-treated rats. Effective re-innervation of injured muscles occurred only at the long-term duration.
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BACKGROUND@#Skeletal muscle injuries are frequent clinical challenges due to associated fibrosis and disability.Regenerative medicine is an emerging promising strategy for such cases. The aim of this study was to compare between the effects of bone marrow-mesenchymal stem cells (BM-MSCs) versus adipose tissue stromal cells (ADSCs) on regeneration and re-innervation of skeletal muscle laceration injury in Wistar rats at different time intervals. @*METHODS@#Six young male rats were used as a source of allogenic MSCs. Eighty-four adult female rats were divided into: Group I (control), Group II (Untreated Laceration): right gluteal muscle was lacerated and left for spontaneous healing, Group III (BM-MSCs): right gluteal muscle was lacerated with concomitant local intramuscular injection of 1 x 106 BM-MSCs in the lacerated muscle, Group IV (ADSCs): right gluteal muscle was lacerated with concomitant local intramuscular injection of 1 x 106 ADSCs in lacerated muscle. Rats were sacrificed after one, two and eight weeks.Muscles were processed to prepare sections stained with H&E, Mallory’s trichrome and immune-histochemical staining (neurofilament light chain). @*RESULTS@#A significant increase in collagen fibers and failure of re-innervation were noticed in untreated laceration group. BM-MSCs-treated groups showed regeneration of muscle fibers but with increased collagen fibers. Meanwhile, ADSCs showed better regenerative effects evidenced by significant increase in the number of myotubes and significant decrease in collagen deposition. Re-innervation was noticed in MSCs-injected muscles after 8 weeks of laceration. @*CONCLUSION@#Both BM-MSCs and ADSCs improved regeneration of skeletal muscle laceration injury at short- and long-term durations. However, fibrosis was less in ADSCs-treated rats. Effective re-innervation of injured muscles occurred only at the long-term duration.
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OBJECTIVES: In Egypt, the National Cancer Registry Program integrates hospital-based data from multiple Egyptian governorates to obtain representative rates. Unfortunately, Dakahlia (one of the largest Egyptian governorates) was not integrated in the National Cancer Registry Program. This research aimed to acquire malignancy rates from the Oncology Center of Mansoura University, which is one of the two oncology centers present in Dakalia Governorate in Egypt. METHODS: Electronic records of patients who attended the Oncology Center of Mansoura University during 2016 were accessed with permission. Analysis was performed to extract diagnostic categories (age, gender, and geographic distribution of cases). RESULTS: Most commonly diagnosed malignancies were breast cancer which represented about 10% of cases in the Oncology Center of Mansoura University during 2016. This was followed by leukemia (3.80%), lymphoma (3.59%), and liver cancer (3.44%). Diagnoses encountered included benign and malignant tumors as well as non-tumor diagnoses. The Mansoura district had the highest proportionate rate of breast cancer cases. Females in the age group ≥ 35 < 60 years had the highest incidence of malignancy cases across all diagnoses. CONCLUSION: The burden of breast cancer in Mansoura district is high. Risk factors need further evaluation with a recommendation to perform an environmental risk assessment.
Assuntos
Feminino , Humanos , Neoplasias da Mama , Estudos Transversais , Diagnóstico , Egito , Incidência , Leucemia , Neoplasias Hepáticas , Linfoma , Medição de Risco , Fatores de RiscoRESUMO
We decreased a simple procedure in treating congenital and cicatricial membranes in the anterior part of the larynx. This procedure can be applicable in children and adults. A sialastic keel is placed between the anterior portions of both vocal cords after endoscopic section of the membrane. The procedure was successful in four properly selected cases
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Humanos , EndoscopiaRESUMO
This study includes 24 paediatric patients with normal healthy ears. They were divided into 2 groups. In group I [18 patients], the maintenance of anaesthesia was achieved with halothane in N2O / Shibin El-Kom while in Group II [6 patients] halothane in oxygen was used. Middle ear pressure was measured prior to anaesthesia, during operation and post operative. Tympanometry was done ten days after operation. All patients of group I developed a negative pressure in one or both ears during the period of the study. Six patients developed bilateral secretory otitis media, while 4 patients developed secretory otitis media. In this study the relationship between persistent negative pressure developed in the middle ear following nitrous oxide anaesthesia and secretory otitis media in children is determined
Assuntos
AnestesiaRESUMO
Extracorporeal shock wave lithotripsy [ESWL] is a new line of treatment of the urinary calculi. Sound created by the Dornier HM-3 lithotriptor was found to be of sufficient intensity to cause hearing impairment. In this study, four operating room personnel [two engineers and two technicians] were exposed to the impulses created by HM-3 lithotriptor. They had normal E.N.T. examination and audiograms before they started to work in the ESWL unit. Pure tone audiometry was done for all personnel after intervals of 6 months, one year and two years. We observed that all personnel suffered from bilateral sensorineural hearing loss [S.N.H.L.] after one year. Thechnicians audiograms showed more hearing impairment than those of engineers. The technicians were exposed to more acoustic trauma. After two years, there is little increase of S.N.H.L. Furthermore, in an audiogram done after a three month interval of rest away from the ESWL unit, considerable hearing improvement had occurred. But there is still some hearing loss. Hearing protection is recommended for operating room personnel